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Ochratoxin Immunoaffinity Column

45-16 Ramsey Road,, Shirley, New York, 11967 (USA)

phone: 16316244882 | fax: 16316147828

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About Ochratoxin Immunoaffinity Column

HPLC: Shimadzu
Column: Trentec Reprosil-Pur RP C18 120 ODS3 5μm; 125x3,0mm with guard column
Mobile Phase A: acetonitrile / deion. water (70/30, v/v)
Mobile Phase B: 0.03 M sodium acetate (pH=4,0) / acetonitrile (65/35 v/v)
Gradient: 0,01 min B 100 %; 10 min B 100 %; 11 min B 0 %; 13 min B 0 %; 15 min B 100 %;
Flow Rate: 0.5ml/min
Time of Analysis: 25min
Injector Volume: 100μl
Fluorescence-Detection: λEX [nm]: 333nm; λEX EM [nm]: 460nm
Temperature: Machine and eluents are at room temperature
Eluents are degassed with helium gas.
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